TRICARBOXYLIC ACID CYCLE IN PSEUDOMONAS AERUGINOSA

Tricarboxylic acid cycle in Pseudomonas aeruginosa.

During the past few years scattered information has appeared which indicates that at least some bacteria probably possess a tricarboxylic acid cycle. Lockwood and Stodola found that Pseudomonas jluorescens formed considerable quantities of a-ketoglutaric acid when growing on a glucose medium under conditions of intense aeration (1). Ajl and Werkman found evidence for at least parts of the conve...

Tricarboxylic Acid Cycle in Pseudomonas Aeruginosa”

During the past few years scattered information has appeared which indicates that at least some bacteria probably possess a tricarboxylic acid cycle. Lockwood and Stodola found that Pseudomonas jluorescens formed considerable quantities of a-ketoglutaric acid when growing on a glucose medium under conditions of intense aeration (1). Ajl and Werkman found evidence for at least parts of the conve...

Evidence for the occurrence of Permeases for tricarboxylic acid cycle intermediates in Pseudomonas aeruginosa.

Washed suspensions and cell-free extracts of Pseudomonas aeruginosa grown on Lemco agar, to which intermediates of the tricarboxylic acid cycle had been added, were tested for their ability to oxidize succinate, fumarate, malate, pyruvate, acetate, a-oxoglutarate and citrate. Whole organisms had lag periods of 2-3 hr. before citrate was oxidized rapidly, except when citrate or acetate had been ...

Tricarboxylic Acid Cycle in

Identification of a multienzyme complex of the tricarboxylic acid cycle enzymes containing citrate synthase isoenzymes from Pseudomonas aeruginosa.

A multienzyme complex of tricarboxylic acid cycle enzymes, catalysing the consecutive reactions from fumarate to 2-oxoglutarate, has been identified in extracts of Pseudomonas aeruginosa prepared by gentle osmotic lysis of the cells. The individual enzyme activities of fumarase, malate dehydrogenase, citrate synthase, aconitase and isocitrate dehydrogenase can be used to reconstitute the comple...